Web18 Nov 2024 · The RNA was then subjected to cDNA synthesis using the PrimeScript first cDNA Synthesis Kit (Takara, Dalian, China). ... Specific primers containing Sac I and Not I restriction enzyme sites were designed to amplify the full fragment encoding the mature protein . The PCR product was purified and inserted into the pEASY-T1 simple cloning … WebUsing this insert restriction enzyme cutting-free cloning (ICFC) ... 500 ng of pSG5 vector (Stratagene, La Jolla, CA, USA) was digested with EcoRI and BglII restriction endonucleases (TaKaRa). The cleaved vector and the two RT-PCR products were separated by 1% agarose gel electrophoresis and purified by Gel Extraction Kit (TaKaRa). The two ...
Primer design and other tools - Mutagenesis kits
Web17 May 2016 · The sequenced amplicon was digested with the restriction endonucleases XbaI and EcoRI and cloned into pBB5-P 0169 vector restricted with the same enzymes, resulting in plasmids pBB5-P 0169-sldAB. This plasmid and as a control pBB5-P 0169 vector were transferred into the desired G. oxydans ZJU2 strain by electroporation and were … Web12 Apr 2024 · Takara Bio: Cat#740609.250: NucleoMag NGS Clean-up and Size Select: Takara Bio: Cat#744970.500: NucleoBond Xtra Midi Plus: Takara Bio: Cat#740412.50: ... respectively. pRA027 was double digested with restriction enzymes MfeI and SacII (New England Biolabs) to integrate RT-SpCas9 in YJM789 (YJM), and selected for … glasgow vs edinburgh scotland
TA cloning: Ultimate guide - Sharebiology
Websmai restriction enzyme ( TaKaRa ) TaKaRa is a verified supplier TaKaRa manufactures this product About News Press Release Team Advisors Partners Contact Bioz Stars Bioz … WebTaKaRa nhei bspei restriction enzymes Nhei Bspei Restriction Enzymes, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed … WebTaKaRa hindiii restriction enzymes Hindiii Restriction Enzymes, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - … fy1 application points