WebSep 10, 2011 · DAPI is a common stain for DNA and shows a shift from a blue DNA-specific signal with an emission maximum around 460 nm to a yellow signal with an emission maximum around 525 nm when binding to polyphosphate (Tijssen et al. 1982). WebDAPI (4′,6-diamidino-2-phenylindole), a widely used fluorescent label for DNA, also interacts with polyphosphate. Binding of poly-P to DAPI, shifts its peak emission wavelength …
High sensitivity, quantitative measurements of polyphosphate using …
WebJan 1, 1999 · Polyphosphate-accumulating bacteria in activated sludge samples from an anaerobic/aerobic batch-type reactor for enhanced biological phosphate removal (EBPR) were identified by dual staining with ribosomal RNA-targeted oligonucleotide probes and 4′,6-diamidino-2-phenylindol dihydrochloride (DAPI) at a polyphosphate-probing … WebApr 4, 2024 · 4′6-diamidino-2-phenylindole (DAPI) 13 stained filaments from the first three days (0, 24 and 72 h), during which most of the phosphorus uptake occurred, gave a first impression of the overall... bitham mill westbury
Accumulation of phosphate and polyphosphate by - OUP …
WebAug 27, 2024 · Polyphosphate and heavy metal resistance in fungi. The yeast species differ in heavy metal tolerance. For example, Candida tropicalis from wastewater was resistant to Zn 2+, Ni 2+, Hg 2+, and Pb 2 (Rehman and Anjum 2011). Cryptococcus humicola was resistant to Cd 2+, Co 2+, Ni 2+ and Mn 2+ (2.5 mM), while the growth of … WebCounterstaining protocol. Equilibrate the sample briefly with phosphate-buffered saline (PBS). Dilute the DAPI stock solution to 300 nM in PBS. Add approximately 300 µL of this dilute DAPI staining solution to the coverslip preparation, making certain that the cells are completely covered. Incubate for 1–5 minutes. WebSep 1, 2012 · The cells accumulated P i mostly in the form of polyphosphate (PolyP). In the presence of Mg 2+, the content of PolyP with longer average chain length increased in both yeasts; they both had numerous inclusions fluorescing in the yellow region of the spectrum, typical of DAPI-PolyP complexes. data analysis with python roadmap